Articles

Effects of <em>Pinus taeda</em> leaf anatomy on vascular and extravascular leaf hydraulic conductance as influenced by N-fertilization and elevated CO<sub>2 </sub>

Abstract

Silvicultural practices (e.g., nitrogen addition through fertilization) and environmental changes (e.g., elevated [CO2]) may alter needle structure, impacting mass and energy exchange between the biosphere and atmosphere through alteration of stomatal function. Hydraulic resistances in leaves, controlling the mass and energy exchanges, occur both in the xylem and in the flow paths across the mesophyll to evaporation sites, and therefore largely depends on the structure of the leaf. We used the Free-Air Carbon dioxide Enrichment (FACE) experiment, providing a unique setting for assessing the interaction effects of [CO2] and nitrogen (N) supply to examine how leaf morphological and anatomical characteristics control leaf hydraulic conductance (Kleaf) of loblolly pine (Pinus taeda L.) trees subjected to ambient or elevated (+200 ppmv) CO2 concentrations (CO2a and CO2e, respectively) and to soil nitrogen amendment (N). Our study revealed that CO2e decreased the number of tracheids per needle, and increased the distance from the xylem vascular bundle to the stomata cavities, perturbing the leaf hydraulic system. Both treatments induced a decrease in Kleaf, and CO2e also decreased leaf extravascular conductance (Kextravascular), the conductance to water flow from the xylem to the leaf-internal air space. Decline in Kleaf under CO2e was driven by the decline in Kextravascular, potentially due to longer path for water movement through the mesophyll, explaining the decline in stomatal conductance (gs) observed under CO2e. This suggests that the distance from vascular conduits to stomata sub-cavity was a major constraint of leaf water transport. Across treatments our results showed that needle vein conductivity was slightly more limited by the lumen than by the bordered-pits, the latter accounting for 30-45% of vein resistance. CO2e-induced reduction in Kleaf was also consistent with an increased resistance to xylem collapse due to thicker cell wall. In addition, stomatal closure corresponded to the water potential inducing a reduction in 50% of leaf vascular conductance (Kvascular) via xylem wall rupture. The water potential that was estimated to induce complete xylem wall collapse was related to the water potential at turgor loss. Our study provided a framework for understanding the interaction between CO2e and N availability in affecting leaf anatomy, and the mechanisms for the response of Kleaf to the treatments. These mechanisms can be incorporated into predictive models of gs, critical for estimating forest productivity in water limited environments in current and future climates and a landscape composed of sites of a range in soil N fertility. 

Introduction

Water flow in the soil-plant-atmosphere continuum is determined by the whole-plant hydraulic conductance from soil through plant tissues (Kplant), characterizing the structural capacity of the plant for water flow (Zimmermann 1983). Changes in Kplant related to changes in size (McCulloh et al. 2015), drought (Meinzer et al. 2003, Brodribb and Holbrook 2003, Addington et al. 2004, Domec and Pruyn 2008), fertilization (Bucci et al. 2006), elevated CO2 (CO2e) concentration (Domec et al. 2010) have been linked to variation in gas exchange, carbon uptake and growth. For the last fifteen years, plant physiologists have come to appreciate the influence that leaves make to Kplant, and their importance to understanding scaling physiological processes within plants (Brodribb and Holbrook 2004, Sacks and Holbrook 2006, Johnson et al. 2011, McCulloh et al. 2015).

Past studies showed that, in Pinus taeda, leaf hydraulic conductance (Kleaf) was strongly correlated with Kplant among trees exposed to a range in resource availabilities imposed by soil nitrogen amendment (N) and CO2e (Domec et al. 2009a, Tor-ngern al. 2015). Because leaves represented a substantial component of Kplant, reduction in Kleaf under CO2e appeared to impose a decline in the efficiency of water conduction through the plant, thus affecting leaf gas exchange and tree transpiration over the long term. In P. taeda, a recent short-term manipulations in CO2e caused no direct response in stomatal functioning, and the observation of long-term 21% reduction in stomatal conductance (gs) was consistent with the indirect effect of decreased Kleaf and increased mutual shading, the latter associated with greater leaf area index (Tor-ngern al. 2015). The differences in the CO2e x fertilized response of Kleaf may potentially be related to the characteristic differences in the development of conducting tissue effects of the treatments on plant ontogeny (Pritchard et al. 1999Lin et al. 2001). Although leaf morphological features have been assessed for their contribution to plant responses to N-fertilization and CO2e (Thomas and Harvey 1991, Lin et al. 2001, Maier et al. 2008), information on such responses is still limited. The responses of leaf anatomy to rising CO2e and to fertilization has already been quantified, but most studies have focused on stomatal structure and density (Radoglou and Jarvis 1990, Woodward and Kelly 1995, Reid et al. 2007), and no data are available on anatomical characteristics of the leaf xylem of trees growing under future CO2 conditions, especially in relation to Kleaf.

Xylem structure determines the hydraulic efficiency and safety of a particular plant organ (Zimmermann 1983, Hacke et al. 2005), providing the structural basis for gas exchange regulation. In leaves, water exits the xylem veins through the bundle sheath cells and then across the endodermis to the mesophyll where it evaporates and then diffuses through the stomata (Leegood 2008). In conifers, the needle vein architecture is very simple, consisting of a single unbranched strand of xylem and phloem surrounded by transfusion tissue. The vascular tissues are separated from the chlorophyll-containing mesophyll by an endodermis consisting of casparian strips. Leaf hydraulic conductance measures the contribution of both vascular and extravascular pathways for water movement inside leaves. Several studies have revealed that the hydraulic resistances of the vascular and extravascular compartments are of the same order of magnitude and vary depending on species and environmental conditions (Zwieniecki et al. 2002, Sack and Tyree 2005, Nardini and Salleo 2005). Reduction in Kleaf following a decline in leaf water status, have been shown to reflect a reduction in the leaf vascular hydraulic conductance (Kvascular) via xylem cavitation in veins (Bucci et al. 2003, Nardini et al. 2003, Johnson et al. 2009, 2012). However, other studies showed that the reduction in Kleaf was more of a consequence of the reduction in extravascular leaf hydraulic conductance (Kextravascular) (Charra-Vaskou et al. 2012, Scoffoni 2015, Bouche et al. 2016). Variations in transfusion tissue, mesophyll area, and distance from vascular conduits to stomata sub-cavity is viewed as a possible determinant of Kextravascular (Aasamaa et al. 2001, Sack et al. 2003, Brodribb et al. 2007). Yet, it is still unclear which leaf structural features drive the functional differences in Kextravascular and Kvascular among treatments (Buckley 2015, Sack et al. 2015). These data may be necessary to discriminate between the responses of leaf mesophyll and vascular tissues to CO2e, and thus for bridging data collected at fine, physiological level to whole-plant processes. In angiosperms, Kvascular can be determined by cutting subsequent vein orders until the increase of Kleaf converges on the expected vascular conductance (Nardini and Salleo 2005, Coomes et al. 2008). For conifers, the lack of distinct veins requires an alternative method which disrupts the living structures of the leaf by freezing or boiling the entire organ. The measured conductance is then reduced to its vascular component (Tyree and Nardini 2001, Cochard et al. 2004a).

Tracheid anatomical data can also be useful in determining hydraulic failure by xylem wall collapse (implosion) under tension, because it is simply a function of tracheid cell wall thickness in relation to the lumen diameter (Hacke et al. 2001, Pittermann et al. 2006, Rosner et al. 2016). In lignified plant organs, simple calculations have suggested that the pressure differentials between adjacent water- and air-filled conduits are not sufficient to cause rupture of lignified cell walls (Rundel and Stecker 1977, Jacobsen et al. 2005, Domec et al. 2009b). Because leaves possess numerous air space and experience large pressure differentials across cell walls, a decrease of Kvascular in dehydrating pine needles due to tracheid collapse has been shown to be plausible (Cochard et al. 2004b), although a recent study showed otherwise with deformation of tracheid walls occurring only in seedlings, but not in matures trees (Bouche et al. 2016). Wall collapse reduces lumen area and considerably increases tracheid hydraulic resistance to water flow, thus impacting Kvascular (Brodribb and Holbrook 2005). Based on these considerations, if leaf anatomical traits adjust to variation in resource availabilities (CO2e and/or N-fertilization), this should be reflected in changes in leaf hydraulic capabilities and consequently in gs.

Long-term treatments of CO2e and N-fertilization perturbed the hydraulic system, allowing a test of how broadly influences of leaf structural characteristics hold outside the range found under of typical conditions. We investigated the structural and anatomical basis for the decreased in Kleaf and its higher sensitivity to leaf water potential in the CO2e x N-fertilized trees. Specifically, we hypothesize that: 1) acclimations in xylem vascular structure and, thus, in Kvascular dominates the difference in Kleaf under CO2e and/or N-fertilization; 2) lower Kleaf in trees growing under CO2e and N is associated with an increased in xylem wall resistance to collapse, and the water potential at turgor loss; and 3) variations in mesophyll area and distance from vascular conduits to stomata sub-cavity is correlated with Kextravascular, which also constraints Kleaf and gs. To test these hypotheses we measured needle anatomical and hydraulic properties of trees growing under fertilization and CO2e (Duke free-air CO2 enrichment: FACE) and compared these traits to published values of water potential at turgor loss and gs measured on the same trees (Domec et al. 2009a).

Materials and Methods

Experimental setting

The experiment was located in a loblolly pine (Pinus taeda L.) plantation established in 1983 on low fertility, acidic clay-loam of the Enon series, in the Blackwood Division of Duke Forest, North Carolina (35°58′ N, 79°08′ W). The experimental site consisted of four plots exposed to ambient CO2 (CO2a) and four plots targeted at +200 mmol mol-1 CO2 (CO2e) above current, with half of each plot fertilized with nitrogen (Oren et al. 2001, Schäfer et al. 2002). CO2 enrichment was implemented according to the free-air CO2 enrichment (FACE) protocol throughout the year whenever ambient temperature was above 5 °C and wind speed was below 5 m s-1. In 1994, two 30-m diameter plots were established: the FACE prototype plot (Plot 7) and its adjacent untreated reference plot (Plot 8) were established, three of which received CO2e. The other three enriched plots, and their references began operating in 1996. In 1998, the prototype plot and its reference plot were halved using a ditch and a barrier, and one-half of each has received annual nitrogen fertilization (N) of 11.2 g N m-2 (Oren et al. 2001); the same design was implemented in the rest of the plots in 2005.

Leaf structure and anatomy

Due to the difficulty in sampling upper crown needles from each tower in each of the rings, two upper canopy shoots from one tree per ring were sampled for a total of eight current-year needles for each treatment combination. Physical needles characteristics measured included needle length, width, thickness and area, as well as needle density and mass per area (Table 1, Supplementary Figure 1). Samples were then oven-dried at 65 °C for 48 h. Fresh needle areas and volume were obtained geometrically (assuming a needle is a third of a cylinder) from dimensions of length and width measured using a digital caliper (series 500 Mitutoyo, Aurora, IL, USA) and a graduated hand lens (Rundel and Yoder 1998). Needle mass per area (g m-2) was determined as the ratio of dry mass to total green needle area. Needle density (g m-3) was calculated as the ratio of dried weight over fresh volume.

Anatomical features were determined from transverse sections made with a sliding microtome and taken in the middle region of the samples. The sections were then stained with aqueous toluidine blue and mounted on a glass slide with glycerin. We photographed the sections at 40x and 400x magnification with a digital camera mounted on a microscope and analyzed the images using the software Image-J 136b (National Institutes of Health, MD, USA). Images taken at 40x were used to determine the circumference of the needles, the area of the mesophyll and the area surrounded by the endodermis cells that comprises the transfusion tissue and the vascular bundle (phloem and xylem). Images taken at 400x were used to determine the number and the size (lumen, cell wall) of each tracheid within the xylem area. To test whether mesophyll area and distance from vascular conduits to stomata sub-cavity was viewed as a possible constraint on Kleaf, the xylem pathway through the leaf mesophyll was quantified by measuring the linear path length from the xylem to stomata as described in Brodribb et al. (2007). Mean, rather than maximum distance from the xylem to the gas exchange surface was used because in conifers, of which stomata are distributed evenly across the needle surfaces, the path length scales with average distance (Brodribb and Jordan 2008).

Table 1: Physical characteristics of needle samples (n = 33 for needle length, width, total area, mass per are and needle density, and n=8 for thickness, cross sectional, mesophyll and endodermis areas) and xylem distance to stomata obtained in the upper crowns of loblolly pine trees, growing under ambient carbon dioxide concentration, elevated and/or N-fertilized conditions (Duke FACE site). Note that the endodermis area corresponds to the areas delimited by the endodermis cells, i.e. the transfusion tissue and the vascular bundle.


    ANOVA
    Ambient–Unfertilized Ambient–Fertilized Elevated–Unfertilized Elevated–Fertilized CO2 N CO2xN
  Length (cm) 17.4 ± 0.6 19.2 ± 0.8 18.0 ± 0.7 19.7 ±0.7 0.74 0.02 0.70
  Width (mm) 1.52 ± 0.02 1.60 ± 0.03 1.49 ± 0.03 1.61 ± 0.03 0.62 <0.01 0.30
  Thickness (mm) 0.81 ± 0.02 0.78 ± 0.03 0.85 ± 0.04 0.82 ± 0.02 0.13 0.37 0.77
  Total surface area (mm2) 4.8 ± 0.1 5.4 ± 0.2 4.7 ± 0.2 5.4 ± 0.2 0.76 0.01 0.56
  Mass per area (g m-2) 79.1± 2.9 81.0± 5.3 81.6 ± 3.5 82.2 ± 6.2 0.78 0.58 0.77
  Density (g cm-3) 0.39 ± 0.01 0.41± 0.02 0.37 ± 001 0.42 ± 0.02 0.85 0.04 0.27
  Cross sectional area (mm2) 0.50 ± 0.01 0.44 ± 0.02 0.48 ± 0.02 0.45 ± 0.02 0.77 0.05 0.57
  Mesophyll area (mm2) 0.34 ± 0.01 0.29 ± 0.01 0.33 ± 0.01 0.30 ± 0.01 0.90 0.02 0.48
  Endodermis area (mm2) 0.15 ± 0.01 0.14 ± 0.01 0.16 ± 0.01 0.15 ± 0.02 0.91 0.34 0.51
  Mesophyll fraction (%) 68 ± 2 67 ± 01 69 ± 1 67 ± 1 0.82 0.62 0.58
  Xylem Fraction (%) 2.4 ± 0.2 2.6 ± 0.3 1.9 ± 0.1 2.6 ± 0.2 0.39 0.07 0.37
  Tracheids per needle 159 ± 9 126 ± 8 117 ± 7 127 ± 19 0.09 0.44 0.18
  Xylem to stomata (mm) 296 ± 10 276 ± 5 346 ± 10 321 ± 16 0.06 0.08 0.84

Analysis of variance (ANOVA) probability values for carbon dioxide concentration (CO2) and N-fertilization treatment (N) are also shown (the probability level P <0.15 was considered to indicate a trend).

Vascular and extravascular leaf conductances

Measurements of leaf hydraulic resistances on a surface area basis (Rleaf) was measured using a high pressure flow meter on current-year needles (HPFM; Tsuda and Tyree 2000). We report here Rleaf rather than Kleaf (=1/Rleaf), because resistances add in series, enabling measured values for shoots to be partitioned into their resistance components. Briefly, apical shoots with current year-needles attached were attached to the HPFM (Dynamax Gen2, IN, USA) using a compression fitting. Deionized, degassed, water filtered at 0.1 mm was forced at a pressure of 0.2-0.3 MPa into the shoots through the leaf, and eventually out of the stomata. Rshoot (MPa s m2 mmol−1) was recorded every 30 s and was shown to increase during the first 3-5 min. Data points were recorded after stable values were reached corresponding to a coefficient of variation <5% for the last 10 readings. Following determination of Rshoot, the needles were removed with clean razor blades at its connection to the woody stem, and the hydraulic resistance re-measured (Rstem). Measurements typically took 10-15 min for an intact shoot, and additional 5-10 min after needle removal. Rleaf was calculated by subtracting Rstem from Rshoot. Rstem never represented more than 7% of Rshoot, indicating that most of the hydraulic resistances were located in the leaves. Rshoot and Rleaf were measured on both control (intact) leaves and on frozen-thawed needles (frozen at -55˚C for 10 min and then thawed for 30 min at +23 ˚C). Freezing the needles removes most of the membrane component of the non-vascular pathways (Tyree et al. 2001, Nardini et al. 2003, Cochard et al. 2004a), and therefore Kleaf of frozen needles represents a good estimate of the hydraulic conductance of the vascular water pathway (Kvascular). Preliminary tests on four shoots showed that Rshoot, once stable, remained so for >20 min, the period during which removing needles and re-measuring were completed. Note that Rshoot determined using dionized water was statistically similar (paired t-test: P=0.19, n=7) to that found using tap water and that illuminating the shoots did not affect Rshoot and thus Rleaf (paired t-test: P=0.45, n=5). Using the ohm (electrical resistance) analogy applied to a hydraulic circuit, extravascular conductance (Kextravascular) was then calculated as: Kextravascular = (1/Kleaf – 1/Kvascular)-1     Equation 1

Vascular lumen and bordered-pit conductivity

Hydraulic conductance of coniferous tracheids is a function of both the tracheid lumen conductance and bordered-pit conductance. Total conductivity of a needle lumen is also determined by the aggregate conductivity through a population of conduits, some transporting water and others not. To account for the disproportionate contribution of large conduits to total flow, the first step was to estimate tracheid diameter distributions for calculation of mean hydraulic diameter (=Σd5/Σd4, where d = individual conduit diameter, Kolb and Sperry 1999). The theoretical lumen xylem conductivity of needle vein on a leaf area basis (Klumen) was calculated according to the Hagen-Poiseuille law using conduit diameter, mean conduit density and the ratio of leaf xylem to leaf area (Lewis and Boose 1995, Woodruff et al. 2008). Total conductivity of needle is determined by the aggregate conductivity along the entire length. The assumption of equal-sized tracheids holds for much of the needle, but diverged to some degree at the very tip. This divergence induces a correction factor of 0.96 (Zwieniecki et al. 2006) that allows estimation of the integrated Klumen based on the size and number of tracheids measured in the middle section of the needle. Modelling predictions have also shown that pit resistance to water flow in tracheids is not necessarily negligible for conduit diameters ranging from 8-12μm and, therefore, even in pine needles a significant proportion of the resistance to water flow may be confined in the end-wall (Hacke et al. 2005). We estimated the specific conductivity of the pits, which represents the parallel conductivity of pits (Kpit) on a leaf area basis (rather than for individual pits). Measured vascular conductivity was assumed to represent the joint conductivity of both lumen and pits (Klumen and Kpit, respectively) and thus Kpit could be calculated as: Kpit = (1/Kvascular – 1/Klumen)-1     Equation 2

Xylem-wall collapse of vascular bundle conduits

Embolized conduits experience large bending stresses that could lead to cell collapse when water potential drops. Wall collapse reduces lumen area and accordingly considerably increases the hydraulic resistance of tracheid to water flow and Kvascular. The conduit thickness-to-span ratio is typically analyzed as a measure of this vulnerability to xylem-wall collapse (Kennedy and Venard 1962). The basis for this relationship is that, the double xylem cell wall shared by adjacent cells behaves in a manner similar to a long plate of width b (tracheid diameter) and thickness t (double cell-wall thickness), and this plate will buckle under a force proportional to (t/b)2 (Hacke et al. 2001). For needle conduits that are circular in shape, it has been proposed that xylem wall collapse is actually proportional to (t/b)3 (Timoshenko and Gere 1961, Brodribb and Holbrook 2005). The shape of the larger cells constituting the vascular bundles were rather square in shape, whereas the smaller tracheids were indeed more cylindrical. Therefore, because we were also interested in tracheids with diameters within 10% of the mean hydraulic diameter, we calculated collapsing pressures for those large tracheids as a function of (t/b)2, and for the others as a function of (t/b)3. The use of tracheids of mean hydraulic diameter as a measure vulnerability to xylem wall collapse is useful because it is this segment of the tracheid population that represent 50% of conductivity (Kolb and Sperry 1999), and thus by collapsing would induce a 50% reduction in Kvascular.

Statistical analysis

The effects of CO2e and N-fertilization on structural an anatomical feature were tested based on an analysis of variance (ANOVA) based on a split-plot design where individual plots were used as replicates. CO2 concentration and N were the main and split-plot effects, respectively. Probability levels P<0.05, P<0.10 and P<0.15 were considered to indicate a significant effect, a strong tendency, or a trend, respectively. Statistical analyses were performed using SAS (version 9.1, Cary, NC), and curve fits were performed using SigmaPlot (version 12.5, Systat Software, Inc. San Rafael, CA).

Results and Discussion

Our results showed that resource availability affected Kleaf by causing differences in leaf xylem anatomy (Pritchard et al. 1998). As previously reported (Maier et al. 2008), CO2e had no effect on needle length and width and only a marginal effect on thickness (Table 1). As expected (Chandler and Dale 1996), N-Fertilization increased needle length by 9% and fascicle width by 6%, which translated into a significant increase in total needle surface area (Table 1). Leaves were denser and longer in N-fertilized trees (Table 1). Greater leaf hydraulic conductance (Kleaf) under CO2a was not associated with greater xylem cross-sectional area, nor lower mass per area. Transverse section of pine needles revealed that the single central vein had two strands of phloem and xylem embedded in transfusion tissue that represented around 15% of the whole surface area across treatments (Table 1, Supplementary Figure 1). In our transverse sections, the chlorophyll-containing mesophyll comprised 68% of the total surface and was not influenced by any of the treatments (Lin et al. 2001). Mesophyll area and needle area were highly correlated, and a common relationship was observed with a slope of 0.70±0.09 across treatments (Figure 1). Overall, needle tracheid sizes ranged from a minimum diameter of 7µm to a maximum of 12µm, with N-fertilization decreasing the mean tracheid diameter by 8% (Table 2), and CO2e having a negative discernable effect on the of number tracheids per needle (Table 2; Figure 2A). Tracheid wall thickness and tracheid diameter were positively correlated across treatments (r2 = 0.61, P < 0.001) and there was a discernable CO2e and a CO2e×N interaction effect on cell wall thickness (Table 2, Figure 2B). The distance from the xylem bundle to the stomata increased with CO2e, and decreased with N fertilization; however, no interaction was observed (Table 1, Figure 2C). As previously reported (Ceulemans et al. 1994), the mean hydraulic diameter of leaf tracheids and the corresponding tracheid lumen conductivity did not vary among treatments (Table 2).

Table 2: Anatomical characteristics of needle xylem (n=9) obtained in the upper crowns of loblolly pine trees, growing under ambient carbon dioxide concentration, elevated and/or N-fertilized conditions (Duke FACE site). Theoretical tracheid lumen hydraulic conductance based on the Hagen-Poiseuille’s equation (Klumen) and xylem tensions potentially inducing xylem (tracheids) wall collapse are also given.


  ANOVA
  Ambient–Unfertilized Ambient–Fertilized Elevated–Unfertilized Elevated–Fertilized CO2 N CO2xN
Cell wall thickness (mm) 1.25 ± 0.02 1.15 ± 0.05 1.33 ± 0.05 1.39 ± 0.08 0.04 0.78 0.06
Hydraulic diameter (mm) 8.5 ± 0.2 7.7 ± 0.1 8.5 ± 0.2 8.0 ± 0.3 0.38 0.02 0.37
Klumen (mmol m2 s-1 MPa-1) 15.8 ± 2.9 15.3 ± 2.8 19.9 ± 2.0 16.2 ± 2.8 0.43 0.31 0.41
Xylem collapse pressure (MPa) -2.14 ± 0.11 -2.27 ± 0.18 -2.43 ± 0.15 -3.00 ± 0.30 0.07 0.07 0.22
Xylem collapse pressure inducing
50% loss of Kvascular (MPa)
-1.37 ± 0.07 -1.49 ± 0.11 -1.61 ± 0.08 -1.89 ± 0.11 0.03 0.04 0.29
Kleaf (mmol m2 s-1 MPa-1) 5.8 ± 0.5 5.3 ± 0.3 4.9 ± 0.3 4.0 ± 0.4 0.07 0.04 0.42
Kvascular (mmol m2 s-1 MPa-1) 12.4 ± 1.3 10.8 ± 0.2 14.6 ± 2.1 9.8 ± 0.4 0.71 0.03 0.19
Kextravascular (mmol m2 s-1 MPa-1) 10.8 ± 1.0 10.7 ± 1.5 8.3 ± 1.0 6.9 ± 0.9 0.04 0.53 0.56
% Kextravascular 54 ± 2 51 ± 4 62 ± 6 59 ± 2 0.03 0.56 0.99

Analysis of variance (ANOVA) probability values for carbon dioxide concentration (CO2) and N-fertilization treatment (N) are also shown.

Figure 1: Relationship between (A) mesophyll area and needle area, and between (B) mesophyll area and endodermis area of Pinus taeda (loblolly pine) needles grown under elevated CO2 and under N-fertilization. Note: area delimited by the endodermis corresponds to the area surrounded by the endodermis cells, i.e. the transfusion tissue and the vascular bundle (See Supplementary Figure 1). A regression line is drawn for each treatment. The slopes of the relationships between mesophyll area and needle area ranged from 0.79 in ambient CO2, to 0.69 in ambient CO2-fertilized, 0.89 in elevated CO2 and 0.65 in elevated CO2-fertilized. All intercepts were not significantly different from zero. In (A), no differences were found in the slopes of the regression lines. In (B), ambient leaves had a shallower slope (P=0.036) than the other three treatments (Tukey–Kramer test).

Figure 2: Treatment mean of physical and anatomical parameters of Pinus taeda (loblolly pine) needles described in Tables 1 and 2. Variables are expressed in relation to N-fertilization treatment on the x-axis. Means accompanied by different letters indicate a significant effect (least square means significant difference, P < 0.05). Error bars represent standard error of the mean. Trends for Kvascular and for the Tracheid Collapse Pressure inducing 50% loss of Kvascular followed the same trends as %Kextravascular (panel F) and Tracheid Collapse Pressure (Panel D), respectively.

Consistent with earlier observations at the site (Domec al. 2009a), CO2e and N had a strong negative effect on maximum Kleaf (Table 2, Figure 2E); however, no interaction was discernable. The freezing method was effective for determining Kextravascular, but, contrary to our first hypothesis, treatment varied in the proportion of Kvascular to Kleaf; 51% (CO2a) to 64% (CO2e) of the hydraulic resistances were located outside the vascular bundle (Table 2, Figure 3A). The decline in Kleaf under CO2e was driven by the decline in Kextravascular rather than a decline in Kvascular (Figure 4). It is interesting to note that the reduction in Kleaf under CO2e was roughly on the same order of magnitude as the increase in the contribution of Kextravascular, meaning that in absolute terms Kvascular did not vary with CO2e (Table 2, Figure 4).

Following our second hypothesis, the decline of Kextravascular under CO2e explained the decline of stomatal conductance (gs) under CO2e (Figure 3B). The underlying causes of smaller gs under CO2e has been elusive (Domec et al. 2010, Ward et al. 2013), until recently when Tor-ngern al. (2015) demonstrated an indirect effect of CO2e on gs. Regardless of soil moisture or leaf water status, CO2e was shown to induce a 25% reduction in Kleaf of Pinus taeda, which was large enough to explain the observed long term reduction in gs. Treatment-induced adjustments increased the path length of water movement from the xylem bundle to the stomata (Table 2), therefore potentially explaining the increase in resistance along radial extravascular hydraulic pathway (Brodribb et al. 2007, Buckley et al. 2011). This physical constrain to water movement from the xylem to the stomata under CO2e may be the mechanistic explanation for the indirect effect of CO2e on gs (Tor-ngern al. 2015), a previously unrecognized acclimation to changing climate.

Figure 3: (A) Proportion of vascular (xylem) and extravascular (outside xylem) hydraulic resistances to the overall leaf hydraulic resistance (1/Kleaf) and (B) reference stomatal conductance (gs at a vapor pressure deficit of 1 kPa, taken from Domec et al. 2009a) versus the extravascular hydraulic conductance in Pinus taeda (loblolly pine) needles grown under elevated CO2 and under N-fertilization.

Figure 4: Relationship between leaf hydraulic conductance (Kleaf) and vascular (Kvascular) and extravascular (Kextravascular) hydraulic conductances of Pinus taeda (loblolly pine) needles grown under elevated CO2 and under N-fertilization. A regression line is drawn for each treatment. For the relationships between Kleaf and Kextravascular no difference was found in slopes of regression lines between treatments (Tukey–Kramer test).

A hydraulic model suggested that, species with a leaf hydraulic design limited by Kextravascular should be better suited to supply water evenly throughout the leaf (Cochard et al. 2004a, Buckley et al. 2011), increasing stomata sensitivity to environmental changes (Mott and Buckley 2000). Furthermore, previous studies have suggested that evaporation of water into substomatal cavities occurs mainly in the vicinity of the guard cells (Meidner and Edwards 1975, Tyree & Yianoulis 1980, Dewar 2002). This peristomatal transpiration implies that the water potential difference between the leaf epidermis and the guard cells is proportional to the membrane hydraulic conductivity (Heidecker et al. 2003). It is therefore expected that Kextravascular should decline as water potential falls, describing the effects of leaf dehydration and declining cell turgor on membrane permeability (Pantin et al. 2012, Rockwell et al. 2014). Our previous results showed that Kleaf declines faster as leaf water potential drops under CO2e and N-fertilization (Domec et al. 2009a), which could also be explained by the proportionally greater extravascular resistance to leaf water flow (Table 2, Figure 2F). Faster decline of Kleaf under CO2e and N-fertilization may be the consequence of the bundle sheath and mesophyll cells losing turgor and shrinking, thus mechanically increasing resistance to water flow (Brodribb and Holbrook 2004, Scoffoni et al. 2011). The concept of a turgor-limited passage through the bundle sheath and mesophyll is supported by the correlations between the point of turgor loss and Kleaf (Brodribb and Holbrook 2003, Woodruff et al. 2007, Domec et al. 2009a, Scoffoni et al. 2011).

Hydraulic conductance of the vascular tissues depends on both the lumen conductance and the pit membrane conductance. In some species, pit membrane conductivity of xylem in roots and branches is more limiting in trees growing under CO2e conditions (Domec et al. 2010). Across treatment our results showed that needle vein conductivity was slightly more limited by the conduit lumen than by bordered-pit resistance, because pit resistance accounted for only 30-45% of vein resistance. Overall, pit resistance accounted for less than 17% of Kleaf (Figure 3A). There was no CO2e nor N-fertilization effect on needle pit resistance (P>45). We are unaware of another study that have quantified the contribution of end-wall resistance to the overall leaf resistance to water flow in leaves. The low pit resistance suggested that inter-tracheid bordered-pit membranes in loblolly pine leaves may be composed of a thin torus, a small ratio of torus to pit aperture diameter and a weak cell wall (Hacke et al. 2005, Pittermann et al. 2006, Domec et al. 2008, Bouche et al. 2014). These microscopic cell-wall anatomical features would likely minimize pit resistance to water flow, thus enhancing Kvascular, but they would also increase the risk of air-seeding and xylem wall collapse. Inter-conduit pits allow water flow between vessels, but in conifers they also act as safety valves to prevent the spreading of air from embolized vessels (Domec et al. 2006, Delzon et al. 2010).

Our study revealed that CO2e caused thicker tracheid cell walls and thus a change in collapse pressures (Table 2). Xylem collapse pressures decreased with CO2e, and also with N fertilization, but no interaction effect was observed (Table 2; Figure 2D). Following our third hypothesis, treatment-induced reductions in Kleaf were consistent with increased resistance to xylem-wall collapse (Figure 5A) and, therefore, stomatal closure corresponded to a 50% collapse of needle tracheids (Figure 5B). The calculated collapsing pressures required to reduce significantly Kvascular were also strongly correlated with the measured tensions at which gs in the field began to decline (Figure 5B), suggesting that the collapse of needle tracheids could explain the midday depression in Kleaf and gs. If we assume that leaf resistance comprises up to 40% of the whole-tree resistance (Sack and Holbrook 2006, Domec et al. 2009a), then the pressure inducing 50% loss of Kvascular would lower tree conductance by less than 20%. Therefore, this decline in Kvascular is steep enough to create a hydraulic signal triggering stomata closure without compromising the whole-tree hydraulic integrity and thus without preventing gs from reaching its maximum. The physiological significance of xylem-wall collapse under field condition may be its role as part of the hydraulic signals that trigger stomatal closure (Cochard et al. 2004b). Treatment-induced anatomical adjustments, such as a the decrease in tracheid fraction and an increase in cell-wall thickness under CO2e, are consistent with a significant trade-off in conifer wood structure between attaining greater mechanical reinforcement (i.e. higher thickness:span, or wood density) at the expense of reduced hydraulic efficiency (Pittermann et al. 2006, Domec et al. 2009b, Rosner et al. 2016). It should be noted, however, that our calculated xylem-wall collapse pressures may be overestimated due to the use of imprecise value of single-fiber mechanical properties (Jayne 1959, Mott et al. 2002). In actual tracheids, bordered-pits between adjacent cells create cell wall imperfections, causing an interaction between plasticity and instability, making the failure pressure smaller than the theoretical values (Yeh and Kyriakides 1988, Corradi et al. 2005). Furthermore, anatomical adjustments following CO2e induced an 11-25% increase in cell wall thickness (Table 2) and, thus, in the water potentials that would induce total xylem-wall collapse (Figure 2E). Because thick and stiff cell walls have been shown to reduce turgor loss (Marshall and Dumbroff 1999), this could also explain the lower water potential at turgor loss (Figure 5C). Again, these correlations support the concept of a turgor-limited passage through the bundle sheath and mesophyll (Brodribb and Holbrook 2005, Woodruff et al. 2007, Leigh et al. 2011).

Figure 5: Relationships among hydraulic properties of Pinus taeda (loblolly pine) needles. (A) Leaf hydraulic conductance (Kleaf) and (B) leaf water potential at stomatal closure versus the pressure required to collapse tracheids representing 50% of Kvascular (collapse pressure inducing a 50% reduction in Kvascular). (C) Shows the relationship between leaf water potential at turgor loss versus the pressure required to collapse all xylem (mean collapse pressure). Data for Kleaf and leaf water potential at maximum gs as well as data for water potentials at turgor loss are from Domec et al. (2009a).

The strong correlations between Kleaf and xylem-wall collapse (Figure 5A) add weight to other studies suggesting that conduit collapse can contribute to decreasing Kleaf as leaf water potential declines (Brodribb and Holbrook 2005, Scoffoni et al. 2014). From Figure 5A, it can be seen that xylem-wall collapse could reduce Kleaf by 45%, via the total decline in Kvascular, which could then mirror the reduction in Kextravascular (described above) as leaf water potentials drops. Although the mechanism explaining the reduction in Kvascular with leaf water potential likely involves cavitation-induced embolism (Woodruff et al. 2007, Johnson et al. 2009), we cannot rule out that leaf xylem might also collapsed at lower water potential. It has been suggested that the collapse of needle tracheids could be an adaptive means of regulating leaf water potential without risking embolism. Being a mechanical process, cell-deformation could be restored elastically as water potentials are relaxed, whereas the refilling embolized conduits is unlikely. Although recent image analyses showed that tracheid deformation could potentially only occur in seedlings (Bouche et al. 2016), a few other studies have experimentally demonstrated that the theoretical collapse pressure for pine needle tracheids and the surrounding transfusion tissue, calculated from equations that describe cell wall resistance to bending and pipe buckling under tension (Timoshenko and Gere 1961, Hacke et al. 2001), corresponded to actual cell collapse observed during leaf dehydration (Cochard et al. 2004b, Brodribb and Holbrook 2005). What is still unclear, however, is why tracheid cell wall in needles is small relative to lumen diameter as compared to other plant organs where it can be up to three times as large (Hacke et al. 2005, Domec et al. 2010). Usually, the smaller the tracheid lumen, the higher the mean collapse pressure. Recently, Zhang et al. (2014) demonstrated that the collapse of transfusion tissue (that is present in all gymnosperms) can also significantly affect Kleaf. Because these cells are in direct contact with the xylem bundle, one can hypothesize a direct link between the collapse of the two adjacent tissues, thus creating a concomitant change in Kvascular and Kextravascular.

Conclusions

Utilizing unique experimental settings, such as FACE, to examine the effect of CO2e on plant structure increases our ability to forecast future environmental impacts on forest growth and function. We showed that long-term acclimation to CO2e altered the structure and anatomy of loblolly pine leaves, impacting the leaf hydraulic system and affecting stomatal conductance. We show evidence that CO2e and N-Fertilization treatments induced a decrease in leaf hydraulic capacity and also decreased the conductance to water flow from the xylem to the leaf-internal air space. This contrasts with previous studies focusing on a direct stomatal response to CO2e. These findings suggest that structural adjustments to CO2e and their consequences to gas exchange must be incorporated in models employed to predict forest responses to climate change.


Acknowledgements : this research was supported by the Office of Science (BER and TES) of the US Department of Energy through the Southeastern Regional Center (SERC) of the National Institute for Global Environmental Change (NIGEC), and through Terrestrial Carbon Processes (TCP) program. This research was also supported by NSF-EAR-1344703 and NSF-IOS-1549971 and grants from the French Research Agency (MACACC ANR-13-AGRO-0005 and MARIS ANR-14-CE03-0007).

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Authors


Jean-Christophe Domec

Affiliation : Bordeaux Sciences Agro, UMR 1391 INRA-ISPA, 33175 Gradignan Cedex, France; Nicholas School of the Environment, Box 90328, Duke University, Durham, NC 27708, USA

jc.domec@agro-bordeaux.fr

Sari Palmroth

Affiliation : Nicholas School of the Environment, Box 90328, Duke University, Durham, NC 27708, USA


Ram Oren

Affiliation : Nicholas School of the Environment, Box 90328, Duke University, Durham, NC 27708, USA

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